SrasV1, Main, Exploration, bibRecord, 001F08

Correlation between TGF‐β1 expression and proteomic profiling induced by severe acute respiratory syndrome coronavirus papain‐like protease

Identifieur interne : 001F08 ( Main/Exploration ); précédent : 001F07; suivant : 001F09

Correlation between TGF‐β1 expression and proteomic profiling induced by severe acute respiratory syndrome coronavirus papain‐like protease

Auteurs : Shih-Wen Li [Taïwan] ; Tsuey-Ching Yang [Taïwan] ; Lei Wan [Taïwan] ; Ying-Ju Lin [Taïwan] ; Fuu-Jen Tsai [Taïwan] ; Chien-Chen Lai [Taïwan] ; Cheng-Wen Lin [Taïwan]

Source :

PROTEOMICS [ 1615-9853 ] ; 2012-11.

RBID : ISTEX:315E3998EFDD7D5375655F657609DE9A9755D6F9

Descripteurs français

Pascal (Inist)
- Coronavirus, Multicatalytic endopeptidase complex, Papain, Protéomique, Syndrome respiratoire aigu sévère, Ubiquitine, Vimentine, Virus syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Coronavirus, Multicatalytic endopeptidase complex, Papain, Proteomics, Severe acute respiratory syndrome, Severe acute respiratory syndrome virus, Ubiquitin, Vimentin.

Abstract

Severe acute respiratory syndrome (SARS) coronavirus (SARS‐CoV) papain‐like protease (PLpro), a deubiquitinating enzyme, demonstrates inactivation of interferon (IFN) regulatory factor 3 and NF‐κB, reduction of IFN induction, and suppression of type I IFN signaling pathway. This study investigates cytokine expression and proteomic change induced by SARS‐CoV PLpro in human promonocyte cells. PLpro significantly increased TGF‐β1 mRNA expression (greater than fourfold) and protein production (greater than threefold). Proteomic analysis, Western blot, and quantitative real‐time PCR assays indicated PLpro upregulating TGF‐β1‐associated genes: HSP27, protein disulfide isomerase A3 precursor, glial fibrillary acidic protein, vimentin, retinal dehydrogenase 2, and glutathione transferase omega‐1. PLpro‐activated ubiquitin proteasome pathway via upregulation of ubiquitin‐conjugating enzyme E2–25k and proteasome subunit alpha type 5. Proteasome inhibitor MG‐132 significantly reduced expression of TGF‐β1 and vimentin. PLpro upregulated HSP27, linking with activation of p38 MAPK and ERK1/2 signaling. Treatment with SB203580 and U0126 reduced PLpro‐induced expression of TGF‐β1, vimentin, and type I collagen. Results point to SARS‐CoV PLpro triggering TGF‐β1 production via ubiquitin proteasome, p38 MAPK, and ERK1/2‐mediated signaling.

Url:

DOI: 10.1002/pmic.201200225

Affiliations:

Taïwan

Le document en format XML

<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Correlation between TGF‐β1 expression and proteomic profiling induced by severe acute respiratory syndrome coronavirus papain‐like protease</title>
<author><name sortKey="Li, Shih En" sort="Li, Shih En" uniqKey="Li S" first="Shih-Wen" last="Li">Shih-Wen Li</name>
</author>
<author><name sortKey="Yang, Tsuey Hing" sort="Yang, Tsuey Hing" uniqKey="Yang T" first="Tsuey-Ching" last="Yang">Tsuey-Ching Yang</name>
</author>
<author><name sortKey="Wan, Lei" sort="Wan, Lei" uniqKey="Wan L" first="Lei" last="Wan">Lei Wan</name>
</author>
<author><name sortKey="Lin, Ying U" sort="Lin, Ying U" uniqKey="Lin Y" first="Ying-Ju" last="Lin">Ying-Ju Lin</name>
</author>
<author><name sortKey="Tsai, Fuu En" sort="Tsai, Fuu En" uniqKey="Tsai F" first="Fuu-Jen" last="Tsai">Fuu-Jen Tsai</name>
</author>
<author><name sortKey="Lai, Chien Hen" sort="Lai, Chien Hen" uniqKey="Lai C" first="Chien-Chen" last="Lai">Chien-Chen Lai</name>
</author>
<author><name sortKey="Lin, Cheng En" sort="Lin, Cheng En" uniqKey="Lin C" first="Cheng-Wen" last="Lin">Cheng-Wen Lin</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:315E3998EFDD7D5375655F657609DE9A9755D6F9</idno>
<date when="2012" year="2012">2012</date>
<idno type="doi">10.1002/pmic.201200225</idno>
<idno type="url">https://api.istex.fr/ark:/67375/WNG-544ZDXNC-K/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000B39</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000B39</idno>
<idno type="wicri:Area/Istex/Curation">000B39</idno>
<idno type="wicri:Area/Istex/Checkpoint">000619</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000619</idno>
<idno type="wicri:doubleKey">1615-9853:2012:Li S:correlation:between:tgf</idno>
<idno type="wicri:source">PMC</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168038</idno>
<idno type="RBID">PMC:7168038</idno>
<idno type="wicri:Area/Pmc/Corpus">001446</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001446</idno>
<idno type="wicri:Area/Pmc/Curation">001446</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001446</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000A78</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000A78</idno>
<idno type="wicri:Area/Ncbi/Merge">003D90</idno>
<idno type="wicri:Area/Ncbi/Curation">003D90</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">003D90</idno>
<idno type="wicri:Area/Main/Merge">001F32</idno>
<idno type="wicri:source">INIST</idno>
<idno type="RBID">Pascal:13-0037501</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000027</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000960</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000063</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000063</idno>
<idno type="wicri:doubleKey">1615-9853:2012:Li S:correlation:between:tgf</idno>
<idno type="wicri:Area/Main/Merge">002014</idno>
<idno type="wicri:Area/Main/Curation">001F08</idno>
<idno type="wicri:Area/Main/Exploration">001F08</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main">Correlation between TGF‐β1 expression and proteomic profiling induced by severe acute respiratory syndrome coronavirus papain‐like protease</title>
<author><name sortKey="Li, Shih En" sort="Li, Shih En" uniqKey="Li S" first="Shih-Wen" last="Li">Shih-Wen Li</name>
<affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung</wicri:regionArea>
<wicri:noRegion>Taichung</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Institute of Molecular Biology, National Chung Hsing University, Taichung</wicri:regionArea>
<wicri:noRegion>Taichung</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yang, Tsuey Hing" sort="Yang, Tsuey Hing" uniqKey="Yang T" first="Tsuey-Ching" last="Yang">Tsuey-Ching Yang</name>
<affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wan, Lei" sort="Wan, Lei" uniqKey="Wan L" first="Lei" last="Wan">Lei Wan</name>
<affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Medical Genetics and Medical Research, China Medical University Hospital, Taichung</wicri:regionArea>
<wicri:noRegion>Taichung</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lin, Ying U" sort="Lin, Ying U" uniqKey="Lin Y" first="Ying-Ju" last="Lin">Ying-Ju Lin</name>
<affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Medical Genetics and Medical Research, China Medical University Hospital, Taichung</wicri:regionArea>
<wicri:noRegion>Taichung</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tsai, Fuu En" sort="Tsai, Fuu En" uniqKey="Tsai F" first="Fuu-Jen" last="Tsai">Fuu-Jen Tsai</name>
<affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Medical Genetics and Medical Research, China Medical University Hospital, Taichung</wicri:regionArea>
<wicri:noRegion>Taichung</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lai, Chien Hen" sort="Lai, Chien Hen" uniqKey="Lai C" first="Chien-Chen" last="Lai">Chien-Chen Lai</name>
<affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Institute of Molecular Biology, National Chung Hsing University, Taichung</wicri:regionArea>
<wicri:noRegion>Taichung</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Medical Genetics and Medical Research, China Medical University Hospital, Taichung</wicri:regionArea>
<wicri:noRegion>Taichung</wicri:noRegion>
</affiliation>
<affiliation></affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">Taïwan</country>
</affiliation>
</author>
<author><name sortKey="Lin, Cheng En" sort="Lin, Cheng En" uniqKey="Lin C" first="Cheng-Wen" last="Lin">Cheng-Wen Lin</name>
<affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung</wicri:regionArea>
<wicri:noRegion>Taichung</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Clinical Virology Laboratory, Department of Laboratory Medicine, China Medical University Hospital, Taichung</wicri:regionArea>
<wicri:noRegion>Taichung</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Biotechnology, Asia University, Taichung</wicri:regionArea>
<wicri:noRegion>Taichung</wicri:noRegion>
</affiliation>
<affiliation></affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">Taïwan</country>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">PROTEOMICS</title>
<title level="j" type="alt">PROTEOMICS</title>
<idno type="ISSN">1615-9853</idno>
<idno type="eISSN">1615-9861</idno>
<imprint><biblScope unit="vol">12</biblScope>
<biblScope unit="issue">21</biblScope>
<biblScope unit="page" from="3193">3193</biblScope>
<biblScope unit="page" to="3205">3205</biblScope>
<biblScope unit="page-count">13</biblScope>
<date type="published" when="2012-11">2012-11</date>
</imprint>
<idno type="ISSN">1615-9853</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">1615-9853</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term>
<term>Multicatalytic endopeptidase complex</term>
<term>Papain</term>
<term>Proteomics</term>
<term>Severe acute respiratory syndrome</term>
<term>Severe acute respiratory syndrome virus</term>
<term>Ubiquitin</term>
<term>Vimentin</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term>
<term>Multicatalytic endopeptidase complex</term>
<term>Papain</term>
<term>Protéomique</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Ubiquitine</term>
<term>Vimentine</term>
<term>Virus syndrome respiratoire aigu sévère</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract">Severe acute respiratory syndrome (SARS) coronavirus (SARS‐CoV) papain‐like protease (PLpro), a deubiquitinating enzyme, demonstrates inactivation of interferon (IFN) regulatory factor 3 and NF‐κB, reduction of IFN induction, and suppression of type I IFN signaling pathway. This study investigates cytokine expression and proteomic change induced by SARS‐CoV PLpro in human promonocyte cells. PLpro significantly increased TGF‐β1 mRNA expression (greater than fourfold) and protein production (greater than threefold). Proteomic analysis, Western blot, and quantitative real‐time PCR assays indicated PLpro upregulating TGF‐β1‐associated genes: HSP27, protein disulfide isomerase A3 precursor, glial fibrillary acidic protein, vimentin, retinal dehydrogenase 2, and glutathione transferase omega‐1. PLpro‐activated ubiquitin proteasome pathway via upregulation of ubiquitin‐conjugating enzyme E2–25k and proteasome subunit alpha type 5. Proteasome inhibitor MG‐132 significantly reduced expression of TGF‐β1 and vimentin. PLpro upregulated HSP27, linking with activation of p38 MAPK and ERK1/2 signaling. Treatment with SB203580 and U0126 reduced PLpro‐induced expression of TGF‐β1, vimentin, and type I collagen. Results point to SARS‐CoV PLpro triggering TGF‐β1 production via ubiquitin proteasome, p38 MAPK, and ERK1/2‐mediated signaling.</div>
</front>
</TEI>
<affiliations><list><country><li>Taïwan</li>
</country>
</list>
<tree><country name="Taïwan"><noRegion><name sortKey="Li, Shih En" sort="Li, Shih En" uniqKey="Li S" first="Shih-Wen" last="Li">Shih-Wen Li</name>
</noRegion>
<name sortKey="Lai, Chien Hen" sort="Lai, Chien Hen" uniqKey="Lai C" first="Chien-Chen" last="Lai">Chien-Chen Lai</name>
<name sortKey="Lai, Chien Hen" sort="Lai, Chien Hen" uniqKey="Lai C" first="Chien-Chen" last="Lai">Chien-Chen Lai</name>
<name sortKey="Lai, Chien Hen" sort="Lai, Chien Hen" uniqKey="Lai C" first="Chien-Chen" last="Lai">Chien-Chen Lai</name>
<name sortKey="Li, Shih En" sort="Li, Shih En" uniqKey="Li S" first="Shih-Wen" last="Li">Shih-Wen Li</name>
<name sortKey="Lin, Cheng En" sort="Lin, Cheng En" uniqKey="Lin C" first="Cheng-Wen" last="Lin">Cheng-Wen Lin</name>
<name sortKey="Lin, Cheng En" sort="Lin, Cheng En" uniqKey="Lin C" first="Cheng-Wen" last="Lin">Cheng-Wen Lin</name>
<name sortKey="Lin, Cheng En" sort="Lin, Cheng En" uniqKey="Lin C" first="Cheng-Wen" last="Lin">Cheng-Wen Lin</name>
<name sortKey="Lin, Cheng En" sort="Lin, Cheng En" uniqKey="Lin C" first="Cheng-Wen" last="Lin">Cheng-Wen Lin</name>
<name sortKey="Lin, Ying U" sort="Lin, Ying U" uniqKey="Lin Y" first="Ying-Ju" last="Lin">Ying-Ju Lin</name>
<name sortKey="Tsai, Fuu En" sort="Tsai, Fuu En" uniqKey="Tsai F" first="Fuu-Jen" last="Tsai">Fuu-Jen Tsai</name>
<name sortKey="Wan, Lei" sort="Wan, Lei" uniqKey="Wan L" first="Lei" last="Wan">Lei Wan</name>
<name sortKey="Yang, Tsuey Hing" sort="Yang, Tsuey Hing" uniqKey="Yang T" first="Tsuey-Ching" last="Yang">Tsuey-Ching Yang</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001F08 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001F08 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     ISTEX:315E3998EFDD7D5375655F657609DE9A9755D6F9
   |texte=   Correlation between TGF‐β1 expression and proteomic profiling induced by severe acute respiratory syndrome coronavirus papain‐like protease
}}

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

Serveur d'exploration SRAS

Correlation between TGF‐β1 expression and proteomic profiling induced by severe acute respiratory syndrome coronavirus papain‐like protease

Correlation between TGF‐β1 expression and proteomic profiling induced by severe acute respiratory syndrome coronavirus papain‐like protease

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.